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Journal of Biomedical Optics

Dynamic contrast-enhanced optical imaging of in vivo organ function
Author(s): Cyrus B. Amoozegar; Tracy Wang; Matthew B. Bouchard; Addason F. McCaslin; Elizabeth M. Hillman; William S. Blaner; Richard M. Levenson
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Paper Abstract

Conventional approaches to optical small animal molecular imaging suffer from poor resolution, limited sensitivity, and unreliable quantitation, often reducing their utility in practice. We previously demonstrated that the in vivo dynamics of an injected contrast agent could be exploited to provide high-contrast anatomical registration, owing to the temporal differences in each organ's response to the circulating fluorophore. This study extends this approach to explore whether dynamic contrast-enhanced optical imaging (DyCE) can allow noninvasive, in vivo assessment of organ function by quantifying the differing cellular uptake or wash-out dynamics of an agent in healthy and damaged organs. Specifically, we used DyCE to visualize and measure the organ-specific uptake dynamics of indocyanine green before and after induction of transient liver damage. DyCE imaging was performed longitudinally over nine days, and blood samples collected at each imaging session were analyzed for alanine aminotransferase (ALT), a liver enzyme assessed clinically as a measure of liver damage. We show that changes in DyCE-derived dynamics of liver and kidney dye uptake caused by liver damage correlate linearly with ALT concentrations, with an 2 value of 0.91. Our results demonstrate that DyCE can provide quantitative, in vivo, longitudinal measures of organ function with inexpensive and simple data acquisition.

Paper Details

Date Published: 6 September 2012
PDF: 6 pages
J. Biomed. Opt. 17(9) 096003 doi: 10.1117/1.JBO.17.9.096003
Published in: Journal of Biomedical Optics Volume 17, Issue 9
Show Author Affiliations
Cyrus B. Amoozegar, Columbia Univ. (United States)
Tracy Wang, Columbia Univ. (United States)
Matthew B. Bouchard, Columbia Univ. (United States)
Addason F. McCaslin, Columbia Univ. (United States)
Elizabeth M. Hillman, Columbia Univ. (United States)
William S. Blaner, Columbia Univ. (United States)
Richard M. Levenson, Univ. of California, Davis (United States)

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