Share Email Print
cover

Journal of Biomedical Optics • Open Access

Noninvasive Raman spectroscopy of rat tibiae: approach to in vivo assessment of bone quality
Author(s): Paul I. Okagbare; Michael D. Morris; Dana Begun; Steven A. Goldstein; Mary Tecklenburg

Paper Abstract

We report on in vivo noninvasive Raman spectroscopy of rat tibiae using robust fiber-optic Raman probes and holders designed for transcutaneous Raman measurements in small animals. The configuration allows placement of multiple fibers around a rat leg, maintaining contact with the skin. Bone Raman data are presented for three regions of the rat tibia diaphysis with different thicknesses of overlying soft tissue. The ability to perform in vivo noninvasive Raman measurement and evaluation of subtle changes in bone composition is demonstrated with rat leg phantoms in which the tibia has carbonated hydroxylapatite, with different carbonate contents. Our data provide proof of the principle that small changes in bone composition can be monitored through soft tissue at anatomical sites of interest in biomedical studies.

Paper Details

Date Published: 6 September 2012
PDF: 3 pages
J. Biomed. Opt. 17(9) 090502 doi: 10.1117/1.JBO.17.9.090502
Published in: Journal of Biomedical Optics Volume 17, Issue 9
Show Author Affiliations
Paul I. Okagbare, Univ. of Michigan (United States)
Michael D. Morris, Univ. of Michigan (United States)
Dana Begun, Univ. of Michigan Medical School (United States)
Steven A. Goldstein, Univ. of Michigan Medical School (United States)
Mary Tecklenburg, Central Michigan Univ. (United States)


© SPIE. Terms of Use
Back to Top