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Journal of Biomedical Optics • Open Access

Optical assessment of tissue anisotropy in ex vivo distended rat bladders
Author(s): Sanaz Alali; Karen J. Aitken; Annette Shroder; Darius J. Bagli; I. Alex Vitkin

Paper Abstract

Microstructural remodelling in epithelial layers of various hollow organs, including changes in tissue anisotropy, are known to occur under mechanical distension and during disease processes. In this paper, we analyze how bladder distension alters wall anisotropy using polarized light imaging (followed by Mueller matrix decomposition). Optical retardance values of different regions of normal rat bladders under different distension pressures are derived. Then optical coherence tomography is used to measure local bladder wall thicknesses, enabling the calculation of the tissue birefringence maps as a measure of the tissue anisotropy. Selected two-photon microscopy is also performed to better understand the compositional origins of the obtained anisotropy results. The dome region of the bladder shows maximum birefringence when the bladder is distended to high pressures, whereas the ventral remains roughly isotropic during distension. In addition, the average anisotropy direction is longitudinal, along the urethra to dome. The derived wall anisotropy trends are based on birefringence as an intrinsic property of the tissue organization independent of its thickness, to aid in understanding the structure-functions relation in healthy bladders. These new insights into the wall microstructure of ex vivo distending bladders may help improve the functionality of the artificially engineered bladder tissues.

Paper Details

Date Published: 17 August 2012
PDF: 9 pages
J. Biomed. Opt. 17(8) 086010 doi: 10.1117/1.JBO.17.8.086010
Published in: Journal of Biomedical Optics Volume 17, Issue 8
Show Author Affiliations
Sanaz Alali, Ontario Cancer Institute (Canada)
Karen J. Aitken, Hospital for Sick Children (Canada)
Annette Shroder, Hospital for Sick Children (Canada)
Darius J. Bagli, Hospital for Sick Children (Canada)
I. Alex Vitkin, Ontario Cancer Institute (Canada)

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