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Journal of Biomedical Optics • Open Access

Fluorescence-based surface magnifying chromoendoscopy and optical coherence tomography endoscope

Paper Abstract

A side-viewing, 2.3-mm diameter, surface magnifying chromoendoscopy-optical coherence tomography (SMC-OCT) endoscope has been designed for simultaneous, nondestructive surface fluorescence visualization and cross-sectional imaging. We apply this endoscope to in vivo examination of the mouse colon. A 30,000 element fiber bundle is combined with single mode fibers, for SMC and OCT imaging, respectively. The distal optics consist of a gradient-index lens and spacer to provide a 1× magnification at a working distance of 1.58 mm in air, necessary to image the sample through a 0.23-mm thick outer glass envelope, and an aluminized right-angle prism fixed to the distal end of the gradient-index lens assembly. The resulting 1∶1 imaging system is capable of 3.9-µm lateral and 2.3-µm axial resolution in the OCT channel, and 125-lp/mm resolution across a 0.70-mm field of view in the SMC channel. The endoscope can perform high contrast crypt visualization, molecular imaging, and cross-sectional imaging of colon microstructure.

Paper Details

Date Published: 3 August 2012
PDF: 8 pages
J. Biomed. Opt. 17(8) 086003 doi: 10.1117/1.JBO.17.8.086003
Published in: Journal of Biomedical Optics Volume 17, Issue 8
Show Author Affiliations
Richard A. Wall, College of Optical Sciences, The Univ. of Arizona (United States)
Jennifer K. Barton, The Univ. of Arizona (United States)

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