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Journal of Biomedical Optics • Open Access

Evaluation of human sclera after femtosecond laser ablation using two photon and confocal microscopy
Author(s): Hui Sun; Ronald M. Kurtz; Tibor Juhasz

Paper Abstract

Glaucoma is the second-leading cause of blindness worldwide and is often associated with elevated intraocular pressure (IOP). Partial thickness intrascleral channels can be created with a femtosecond laser operating at a wavelength of 1700 nm. Such channels have the potential to increase outflow facility and reduce elevated IOP. Analysis of the dimensions and location of these channels is important in understanding their effects. We describe the application of two-photon microscopy and confocal microscopy for noninvasive imaging of the femtosecond laser created partial-thickness scleral channels in human cadaver eyes. High-resolution images, hundreds of microns deep in the sclera, were obtained to allow determination of the shape and dimension of such channels. This demonstrates that concept of integrating femtosecond laser surgery, and two-photon and confocal imaging has the future potential for image-guided high-precision surgery in transparent and translucent tissue.

Paper Details

Date Published: 14 June 2012
PDF: 6 pages
J. Biomed. Opt. 17(8) 081411 doi: 10.1117/1.JBO.17.8.081411
Published in: Journal of Biomedical Optics Volume 17, Issue 8
Show Author Affiliations
Hui Sun, Alcon Labs., Inc. (United States)
Ronald M. Kurtz, Univ. of California, Irvine (United States)
Tibor Juhasz, Univ. of California, Irvine (United States)

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