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Journal of Biomedical Optics

Optical coherence tomography for live phenotypic analysis of embryonic ocular structures in mouse models
Author(s): Irina V. Larina; Saba H. Syed; Mary E. Dickinson; Kirill V. Larin; Narendran Sudheendran; Paul A. Overbeek
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Paper Abstract

Mouse models of ocular diseases provide a powerful resource for exploration of molecular regulation of eye development and pre-clinical studies. Availability of a live high-resolution imaging method for mouse embryonic eyes would significantly enhance longitudinal analyses and high-throughput morphological screening. We demonstrate that optical coherence tomography (OCT) can be used for live embryonic ocular imaging throughout gestation. At all studied stages, the whole eye is within the imaging distance of the system and there is a good optical contrast between the structures. We also performed OCT eye imaging in the embryonic retinoblastoma mouse model Pax6-SV40 T-antigen, which spontaneously forms lens and retinal lesions, and demonstrate that OCT allows us to clearly differentiate between the mutant and wild type phenotypes. These results demonstrate that OCTin utero imaging is a potentially useful tool to study embryonic ocular diseases in mouse models.

Paper Details

Date Published: 14 May 2012
PDF: 5 pages
J. Biomed. Opt. 17(8) 081410 doi: 10.1117/1.JBO.17.8.081410
Published in: Journal of Biomedical Optics Volume 17, Issue 8
Show Author Affiliations
Irina V. Larina, Baylor College of Medicine (United States)
Saba H. Syed, Baylor College of Medicine (United States)
Mary E. Dickinson, Baylor College of Medicine (United States)
Kirill V. Larin, Baylor College of Medicine (United States)
Narendran Sudheendran, Univ. of Houston (United States)
Paul A. Overbeek, Baylor College of Medicine (United States)

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