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Journal of Biomedical Optics • Open Access

Surface plasmon-based infrared spectroscopy for cell biosensing
Author(s): Victor Yashunsky; Vladislav G. Lirtsman; Alexander Zilbershtein; Michael Golosovsky; Dan Davidov; Amir Bein; Betty Schwartz; Benjamin Aroeti

Paper Abstract

Cell morphology is often used as a valuable indicator of the physical condition and general status of living cells. We demonstrate a noninvasive method for morphological characterization of adherent cells. We measure infrared reflectivity spectrum at oblique angle from living cells cultured on thin Au film, and utilize the unique properties of the confined infrared waves (i.e., surface plasmon and guided modes) traveling inside the cell layer. The propagation of these waves strongly depends on cell morphology and connectivity. By tracking the resonant wavelength and attenuation of the surface plasmon and guided modes we measure the kinetics of various cellular processes such as (i) cell attachment and spreading on different substrata, (ii) modulation of the outer cell membrane with chlorpromazine, and (iii) formation of intercellular junctions associated with progressive cell polarization. Our method enables monitoring of submicron variations in cell layer morphology in real-time, and in the label-free manner.

Paper Details

Date Published: 14 June 2012
PDF: 9 pages
J. Biomed. Opt. 17(8) 081409 doi: 10.1117/1.JBO.17.8.081409
Published in: Journal of Biomedical Optics Volume 17, Issue 8
Show Author Affiliations
Victor Yashunsky, The Hebrew Univ. of Jerusalem (Israel)
Vladislav G. Lirtsman, The Hebrew Univ. of Jerusalem (Israel)
Alexander Zilbershtein, The Hebrew Univ. of Jerusalem (Israel)
Michael Golosovsky, The Hebrew Univ. of Jerusalem (Israel)
Dan Davidov, The Hebrew Univ. of Jerusalem (Israel)
Amir Bein, The Hebrew Univ. of Jerusalem (Israel)
Betty Schwartz, The Hebrew Univ. of Jerusalem (Israel)
Benjamin Aroeti, The Hebrew Univ. of Jerusalem (Israel)

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