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Journal of Biomedical Optics • Open Access

Quantitative changes in human epithelial cancers and osteogenesis imperfecta disease detected using nonlinear multicontrast microscopy
Author(s): Javier F. Adur; Vitor B. Pelegati; André A. de Thomaz; Lilia D'Souza-Li; Maria do Carmo Assuncao; Fatima Bottcher-Luiz; Liliana A. Andrade; Carlos Lenz-Cesar

Paper Abstract

We show that combined multimodal nonlinear optical (NLO) microscopies, including two-photon excitation fluorescence, second-harmonic generation (SHG), third harmonic generation, and fluorescence lifetime imaging microscopy (FLIM) can be used to detect morphological and metabolic changes associated with stroma and epithelial transformation during the progression of cancer and osteogenesis imperfecta (OI) disease. NLO microscopes provide complementary information about tissue microstructure, showing distinctive patterns for different types of human breast cancer, mucinous ovarian tumors, and skin dermis of patients with OI. Using a set of scoring methods (anisotropy, correlation, uniformity, entropy, and lifetime components), we found significant differences in the content, distribution and organization of collagen fibrils in the stroma of breast and ovary as well as in the dermis of skin. We suggest that our results provide a framework for using NLO techniques as a clinical diagnostic tool for human cancer and OI. We further suggest that the SHG and FLIM metrics described could be applied to other connective or epithelial tissue disorders that are characterized by abnormal cells proliferation and collagen assembly.

Paper Details

Date Published: 21 May 2012
PDF: 10 pages
J. Biomed. Opt. 17(8) 081407 doi: 10.1117/1.JBO.17.8.081407
Published in: Journal of Biomedical Optics Volume 17, Issue 8
Show Author Affiliations
Javier F. Adur, Univ. Estadual de Campinas (Brazil)
Vitor B. Pelegati, Univ. Estadual de Campinas (Brazil)
André A. de Thomaz, Univ. Estadual de Campinas (Brazil)
Lilia D'Souza-Li, Univ. Estadual de Campinas (Brazil)
Maria do Carmo Assuncao, Locus Lab. (Brazil)
Fatima Bottcher-Luiz, Univ. Estadual de Campinas (Brazil)
Liliana A. Andrade, Univ. Estadual de Campinas (Brazil)
Carlos Lenz-Cesar, Univ. Estadual de Campinas (Brazil)

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