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Journal of Biomedical Optics • Open Access

Theory and practical recommendations for autocorrelation-based image correlation spectroscopy
Author(s): Claire Robertson; Steven C. George

Paper Abstract

Image correlation spectroscopy (ICS) is a powerful technique for detecting arrangement of fluorophores in images. This tutorial gives background into the mathematical underpinnings of ICS, specifically image autocorrelation. The effects of various artifacts and image processing steps, including background subtraction, noise, and image morphology were examined analytically and their effects on ICS analysis modeled. A series of recommendations was built based on this analysis.

Paper Details

Date Published: 8 August 2012
PDF: 8 pages
J. Biomed. Opt. 17(8) 080801 doi: 10.1117/1.JBO.17.8.080801
Published in: Journal of Biomedical Optics Volume 17, Issue 8
Show Author Affiliations
Claire Robertson, Univ. of California, Irvine (United States)
Steven C. George, Univ. of California, Irvine (United States)

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