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Journal of Biomedical Optics • Open Access

Microscopic structural study of collagen aging in isolated fibrils using polarized second harmonic generation
Author(s): Dora Aït-Belkacem; Muriel Roche; Julien Duboisset; Patrick Ferrand; Sophie Brasselet; Marie Guilbert; Ganesh D. Sockalingum; Pierre Jeannesson

Paper Abstract

Polarization resolved second harmonic generation (PSHG) is developed to study, at the microscopic scale, the impact of aging on the structure of type I collagen fibrils in two-dimensional coatings. A ribose-glycated collagen is also used to mimic tissue glycation usually described as an indicator of aging. PSHG images are analyzed using a generic approach of the molecular disorder information in collagen fibrils, revealing significant changes upon aging, with a direct correlation between molecular disorder and fibril diameters.

Paper Details

Date Published: 14 August 2012
PDF: 4 pages
J. Biomed. Opt. 17(8) 080506 doi: 10.1117/1.JBO.17.8.080506
Published in: Journal of Biomedical Optics Volume 17, Issue 8
Show Author Affiliations
Dora Aït-Belkacem, Institut Fresnel (France)
Muriel Roche, Institut Fresnel (France)
Julien Duboisset, Institut Fresnel (France)
Patrick Ferrand, Institut Fresnel (France)
Sophie Brasselet, Institut Fresnel (France)
Marie Guilbert, Univ. de Reims Champagne-Ardenne (France)
Ganesh D. Sockalingum, Univ. de Reims Champagne-Ardenne (France)
Pierre Jeannesson, Univ. de Reims Champagne-Ardenne (France)

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