Share Email Print
cover

Journal of Biomedical Optics • Open Access

Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror

Paper Abstract

By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have to sacrifice either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible microelectromechanical systems (MEMS) scanning mirror (MEMS-OR-PAM). In MEMS-OR-PAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures the uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. Presented results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.

Paper Details

Date Published: 14 August 2012
PDF: 4 pages
J. Biomed. Opt. 17(8) 080505 doi: 10.1117/1.JBO.17.8.080505
Published in: Journal of Biomedical Optics Volume 17, Issue 8
Show Author Affiliations
Junjie Yao, Washington Univ. in St. Louis (United States)
Lidai Wang, Washington Univ. School of Medicine in St. Louis (United States)
Joon-Mo Yang, Washington Univ. in St. Louis (United States)
Liang S. Gao, Washington Univ. in St. Louis (United States)
Konstantin I. Maslov, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)
Chih-Hsien Huang, Texas A&M Univ. (United States)
Jun Zou, Texas A&M Univ. (United States)


© SPIE. Terms of Use
Back to Top