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Journal of Biomedical Optics • Open Access

Mosaic acquisition and processing for optical-resolution photoacoustic microscopy
Author(s): Peng Shao; Wei Shi; Ryan K. Chee; Roger J. Zemp

Paper Abstract

In optical-resolution photo-acoustic microscopy (OR-PAM), data acquisition time is limited by both laser pulse repetition rate (PRR) and scanning speed. Optical-scanning offers high speed, but limited, field of view determined by ultrasound transducer sensitivity. In this paper, we propose a hybrid optical and mechanical-scanning OR-PAM system with mosaic data acquisition and processing. The system employs fast-scanning mirrors and a diode-pumped, nanosecond-pulsed, Ytterbium-doped, 532-nm fiber laser with PRR up to 600 kHz. Data from a sequence of image mosaic patches is acquired systematically, at predetermined mechanical scanning locations, with optical scanning. After all imaging locations are covered, a large panoramic scene is generated by stitching the mosaic patches together. Our proposed system is proven to be at least 20 times faster than previous reported OR-PAM systems.

Paper Details

Date Published: 19 July 2012
PDF: 4 pages
J. Biomed. Opt. 17(8) 080503 doi: 10.1117/1.JBO.17.8.080503
Published in: Journal of Biomedical Optics Volume 17, Issue 8
Show Author Affiliations
Peng Shao, Univ. of Alberta (Canada)
Wei Shi, Univ. of Alberta (Canada)
Ryan K. Chee, Univ. of Alberta (Canada)
Roger J. Zemp, Univ. of Alberta (Canada)

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