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Journal of Biomedical Optics • Open Access

Simultaneous optical coherence tomography and autofluorescence microscopy with a single light source

Paper Abstract

We have accomplished simultaneous spectral domain optical coherence tomography (SD-OCT) and autofluorescence (AF) microscopy with a broadband light source centered at 415 nm. The light source was provided by frequency-doubling of an ultra-fast broadband Ti:Sapphire laser. With a bandwidth of 8 nm, the visible SD-OCT achieved a depth resolution of ∼ 12  μm. Since the two imaging modalities are provided by the same group of photons, their images are intrinsically registered. The dual-modal system is capable of providing OCT imaging and molecular contrasts simultaneously. The imaging system was tested on imaging biological samples ex vivo and in vivo.

Paper Details

Date Published: 13 July 2012
PDF: 4 pages
J. Biomed. Opt. 17(8) 080502 doi: 10.1117/1.JBO.17.8.080502
Published in: Journal of Biomedical Optics Volume 17, Issue 8
Show Author Affiliations
Cuixia Dai, Shanghai Institute of Technology (China)
Xiaojing Liu, The Univ. of Southern California (United States)
Shuliang Jiao, The Univ. of Southern California (United States)


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