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Journal of Biomedical Optics • Open Access

Imaging-guided two-photon excitation-emission-matrix measurements of human skin tissues
Author(s): Yingqiu Yu; Anthony M. Lee; Hequn Wang; Jianhua Zhao; Harvey Lui; Haishan Zeng; Shuo Tang

Paper Abstract

There are increased interests on using multiphoton imaging and spectroscopy for skin tissue characterization and diagnosis. However, most studies have been done with just a few excitation wavelengths. Our objective is to perform a systematic study of the two-photon fluorescence (TPF) properties of skin fluorophores, normal skin, and diseased skin tissues. A nonlinear excitation-emission-matrix (EEM) spectroscopy system with multiphoton imaging guidance was constructed. A tunable femtosecond laser was used to vary excitation wavelengths from 730 to 920 nm for EEM data acquisition. EEM measurements were performed on excised fresh normal skin tissues, seborrheic keratosis tissue samples, and skin fluorophores including: NADH, FAD, keratin, melanin, collagen, and elastin. We found that in the stratum corneum and upper epidermis of normal skin, the cells have large sizes and the TPF originates from keratin. In the lower epidermis, cells are smaller and TPF is dominated by NADH contributions. In the dermis, TPF is dominated by elastin components. The depth resolved EEM measurements also demonstrated that keratin structure has intruded into the middle sublayers of the epidermal part of the seborrheic keratosis lesion. These results suggest that the imaging guided TPF EEM spectroscopy provides useful information for the development of multiphoton clinical devices for skin disease diagnosis.

Paper Details

Date Published: 6 July 2012
PDF: 9 pages
J. Biomed. Opt. 17(7) 077004 doi: 10.1117/1.JBO.17.7.077004
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Yingqiu Yu, The BC Cancer Agency Research Ctr. (Canada)
Anthony M. Lee, The BC Cancer Agency Research Ctr. (Canada)
Hequn Wang, The BC Cancer Agency Research Ctr. (Canada)
Jianhua Zhao, The BC Cancer Agency Research Ctr. (Canada)
Harvey Lui, The Univ. of British Columbia (Canada)
Haishan Zeng, The BC Cancer Agency Research Ctr. (Canada)
Shuo Tang, The Univ. of British Columbia (Canada)

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