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Journal of Biomedical Optics

Visualization of the solubilization process of the plasma membrane of a living cell by waveguide evanescent field fluorescence microscopy
Author(s): Abdollah Hassanzadeh; Heung K. Ma; Silvia Mittler; S. Jeffrey Dixon
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Paper Abstract

Waveguide evanescent field fluorescence microscopy (WEFF) is a novel microscopy technology that allows imaging of a cell's plasma membrane in the vicinity of a glass substrate with high axial resolution, low background and little photobleaching. Time-lapse imaging can be performed to investigate changes in cell morphology in the presence or absence of chemical agents. WEFF microscopy provides a method to investigate plasma membranes of living cells and allows a comparison to simplified model membranes immobilized on planar substrates. The interaction of the nonionic detergent Triton X-100 with plasma membranes of osteoblasts in an aqueous environment was investigated. Solubilization of the membranes very close to the waveguide surface was visualized and related to the three-stage solubilisation model proposed for liposomes and supported lipid bilayers. Findings for the plasma membranes of cells are in excellent agreement with results reported for these artificial model systems.

Paper Details

Date Published: 24 July 2012
PDF: 8 pages
J. Biomed. Opt. 17(7) 076025 doi: 10.1117/1.JBO.17.7.076025
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Abdollah Hassanzadeh, The Univ. of Western Ontario (Canada)
Heung K. Ma, The Univ. of Western Ontario (Canada)
Silvia Mittler, The Univ. of Western Ontario (Canada)
S. Jeffrey Dixon, The Univ. of Western Ontario (Canada)

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