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Journal of Biomedical Optics

Structural alterations in rat liver proteins due to streptozotocin-induced diabetes and the recovery effect of selenium: Fourier transform infrared microspectroscopy and neural network study
Author(s): Ozlem Bozkurt; Feride Severcan; Sevgi H. Bayari; Mete Severcan; Christoph Krafft; Jürgen Popp
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Paper Abstract

The relation between protein structural alterations and tissue dysfunction is a major concern as protein fibrillation and/or aggregation due to structural alterations has been reported in many disease states. In the current study, Fourier transform infrared microspectroscopic imaging has been used to investigate diabetes-induced changes on protein secondary structure and macromolecular content in streptozotocin-induced diabetic rat liver. Protein secondary structural alterations were predicted using neural network approach utilizing the amide I region. Moreover, the role of selenium in the recovery of diabetes-induced alterations on macromolecular content and protein secondary structure was also studied. The results revealed that diabetes induced a decrease in lipid to protein and glycogen to protein ratios in diabetic livers. Significant alterations in protein secondary structure were observed with a decrease in α-helical and an increase in β-sheet content. Both doses of selenium restored diabetes-induced changes in lipid to protein and glycogen to protein ratios. However, low-dose selenium supplementation was not sufficient to recover the effects of diabetes on protein secondary structure, while a higher dose of selenium fully restored diabetes-induced alterations in protein structure.

Paper Details

Date Published: 17 July 2012
PDF: 9 pages
J. Biomed. Opt. 17(7) 076023 doi: 10.1117/1.JBO.17.7.076023
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Ozlem Bozkurt, Middle East Technical Univ. (Turkey)
Feride Severcan, Middle East Technical Univ. (Turkey)
Sevgi H. Bayari, Hacettepe Univ. (Turkey)
Mete Severcan, Middle East Technical Univ. (Turkey)
Christoph Krafft, Institut für Photonische Technologien e.V. (Germany)
Jürgen Popp, Institut für Photonische Technologien e.V. (Germany)

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