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Journal of Biomedical Optics • Open Access

Filter-based method for background removal in high-sensitivity wide-field-surface-enhanced Raman scattering imaging in vivo
Author(s): Rupananda Mallia; Israel Veilleux; Brian C. Wilson; Patrick McVeigh

Paper Abstract

As molecular imaging moves towards lower detection limits, the elimination of endogenous background signals becomes imperative. We present a facile background-suppression technique that specifically segregates the signal from surface-enhanced Raman scattering (SERS)-active nanoparticles (NPs) from the tissue autofluorescence background in vivo. SERS NPs have extremely narrow spectral peaks that do not overlap significantly with endogenous Raman signals. This can be exploited, using specific narrow-band filters, to image picomolar (pM) concentrations of NPs against a broad tissue autofluorescence background in wide-field mode, with short integration times that compare favorably with point-by-point mapping typically used in SERS imaging. This advance will facilitate the potential applications of SERS NPs as contrast agents in wide-field multiplexed biomarker-targeted imaging in vivo.

Paper Details

Date Published: 13 July 2012
PDF: 6 pages
J. Biomed. Opt. 17(7) 076017 doi: 10.1117/1.JBO.17.7.076017
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Rupananda Mallia, Ontario Cancer Institute (Canada)
Israel Veilleux, Ontario Cancer Institute (Canada)
Brian C. Wilson, Ontario Cancer Institute (Canada)
Patrick McVeigh, Univ. of Toronto (Canada)

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