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Journal of Biomedical Optics

Polydimethylsiloxane embedded mouse aorta ex vivo perfusion model: proof-of-concept study focusing on atherosclerosis
Author(s): Xueya Wang; Marc P. Wolf; Rahel Bänziger Keel; Roman Lehner; Patrick Hunziker
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Paper Abstract

Existing mouse artery ex vivo perfusion models have utilized arteries such as carotid, uterine, and mesenteric arteries, but not the aorta. However, the aorta is the principal vessel analyzed for atherosclerosis studies in vivo. We have devised a mouse aorta ex vivo perfusion model that can bridge this gap. Aortas from apoE(-/-) mice are embedded in a transparent, gas-permeable, and elastic polymer matrix [polydimethylsiloxane (PDMS)] and artificially perfused with cell culture medium under cell culture conditions. After 24 h of artificial ex vivo perfusion, no evidence of cellular apoptosis is detected. Utilizing a standard confocal microscope, it is possible to image specific receptor targeting of cells in atherosclerotic plaques during 24 h. Imaging motion artifacts are minimal due to the polymer matrix embedding. Re-embedding of the aorta enables tissue sectioning and immuno-histochemical analysis. The ex vivo data are validated by comparison with in vivo experiments. This model can save animal lives via production of multiple endpoints in a single experiment, is easy to apply, and enables straightforward comparability with pre-existing atherosclerosis in vivo data. It is suited to investigate atherosclerotic disease in particular and vascular biology in general.

Paper Details

Date Published: 6 July 2012
PDF: 9 pages
J. Biomed. Opt. 17(7) 076006 doi: 10.1117/1.JBO.17.7.076006
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Xueya Wang, Basel Univ. Hospital (Switzerland)
Marc P. Wolf, Basel Univ. Hospital (Switzerland)
Rahel Bänziger Keel, Basel Univ. Hospital (Switzerland)
Roman Lehner, Basel Univ. Hospital (Switzerland)
Patrick Hunziker, Basel Univ. Hospital (Switzerland)


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