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Journal of Biomedical Optics • Open Access

Analysis of second-harmonic-generation microscopy in a mouse model of ovarian carcinoma
Author(s): Jennifer M. Watson; Photini F. Rice; Urs Utzinger; Jennifer K. Barton; Samuel L. Marion; Patricia B. Hoyer; Molly A. Brewer; John R. Davis; Jeffrey J. Rodríguez

Paper Abstract

Second-harmonic-generation (SHG) imaging of mouse ovaries ex vivo was used to detect collagen structure changes accompanying ovarian cancer development. Dosing with 4-vinylcyclohexene diepoxide and 7,12-dimethylbenz[a]anthracene resulted in histologically confirmed cases of normal, benign abnormality, dysplasia, and carcinoma. Parameters for each SHG image were calculated using the Fourier transform matrix and gray-level co-occurrence matrix (GLCM). Cancer versus normal and cancer versus all other diagnoses showed the greatest separation using the parameters derived from power in the highest-frequency region and GLCM energy. Mixed effects models showed that these parameters were significantly different between cancer and normal (P<0.008). Images were classified with a support vector machine, using 25% of the data for training and 75% for testing. Utilizing all images with signal greater than the noise level, cancer versus not-cancer specimens were classified with 81.2% sensitivity and 80.0% specificity, and cancer versus normal specimens were classified with 77.8% sensitivity and 79.3% specificity. Utilizing only images with greater than of 75% of the field of view containing signal improved sensitivity and specificity for cancer versus normal to 81.5% and 81.1%. These results suggest that using SHG to visualize collagen structure in ovaries could help with early cancer detection.

Paper Details

Date Published: 4 July 2012
PDF: 10 pages
J. Biomed. Opt. 17(7) 076002 doi: 10.1117/1.JBO.17.7.076002
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Jennifer M. Watson, The Univ. of Arizona (United States)
Photini F. Rice, The Univ. of Arizona (United States)
Urs Utzinger, The Univ. of Arizona (United States)
Jennifer K. Barton, The Univ. of Arizona (United States)
Samuel L. Marion, The Univ. of Arizona (United States)
Patricia B. Hoyer, The Univ. of Arizona (United States)
Molly A. Brewer, Univ. of Connecticut Health Ctr. (United States)
John R. Davis, The Univ. of Arizona (United States)
Jeffrey J. Rodríguez, The Univ. of Arizona (United States)

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