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Journal of Biomedical Optics • Open Access

Deep ultraviolet resonant Raman imaging of a cell

Paper Abstract

We report the first demonstration of deep ultraviolet (DUV) Raman imaging of a cell. Nucleotide distributions in a HeLa cell were observed without any labeling at 257 nm excitation with resonant bands attributable to guanine and adenine. Obtained images represent DNA localization at nucleoli in the nucleus and RNA distribution in the cytoplasm. The presented technique extends the potential of Raman microscopy as a tool to selectively probe nucleic acids in a cell with high sensitivity due to resonance.

Paper Details

Date Published: 3 July 2012
PDF: 5 pages
J. Biomed. Opt. 17(7) 076001 doi: 10.1117/1.JBO.17.7.076001
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Yasuaki Kumamoto, RIKEN (Japan)
Atsushi Taguchi, RIKEN (Japan)
Satoshi Kawata, RIKEN (Japan)
Nicholas I. Smith, Osaka Univ. (Japan)

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