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Journal of Biomedical Optics

Effect of tissue fluid on accurate determination of the complex refractive index of animal tissue
Author(s): Jin Wang; Qing Ye; Zhi-Chao Deng; Wen-Yuan Zhou; Teng-Qian Sun; Chun-Ping Zhang; Jian-Guo Tian
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Paper Abstract

We investigate the effect of tissue fluid on the measurement of complex refractive index (RI) of animal tissue. A new model is proposed and verified through experimental results of simulation samples made of glycerol and methyl-red-doped poly(methyl methacrylate). Coupled with polarized optical reflectance measurements performed on several kinds of animal muscle tissues, RIs were resolved using the new model. We find that the tissue fluid existing at the prism-sample interface is unavoidable. We also find that with a change of proportion of the tissue fluid, the RI of muscle tissue can still be measured using the new model.

Paper Details

Date Published: 17 July 2012
PDF: 7 pages
J. Biomed. Opt. 17(7) 075011 doi: 10.1117/1.JBO.17.7.075011
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Jin Wang, Nankai Univ. (China)
Qing Ye, Nankai Univ. (China)
Zhi-Chao Deng, Nankai Univ. (China)
Wen-Yuan Zhou, Nankai Univ. (China)
Teng-Qian Sun, Nankai Univ. (China)
Chun-Ping Zhang, Nankai Univ. (China)
Jian-Guo Tian, Nankai Univ. (China)

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