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Journal of Biomedical Optics

Morphological and chemical changes in dentin after using endodontic agents: Fourier transform Raman spectroscopy, energy-dispersive x-ray fluorescence spectrometry, and scanning electron microscopy study
Author(s): Fernanda Miori Pascon; Regina Maria Puppin-Rontani; Kamila R. Kantovitz; Luis E. Soares; Ana M. do Espirito Santo; Airton A. Martin
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Paper Abstract

We examine the morphological and chemical changes in the pulp chamber dentin after using endodontic agents by scanning electron microscopy (SEM), Fourier transform Raman spectroscopy (FT-Raman), and micro energy-dispersive x-ray fluorescence spectrometry (μEDXRF). Thirty teeth were sectioned exposing the pulp chamber and divided by six groups (n = 5): NT-no treatment; CHX-2% chlorhexidine; CHXE-2% chlorhexidine+17% EDTA; E-17% EDTA; SH5-5.25% NaOCl; SH5E-5.25% NaOCl+17% EDTA. The inorganic and organic content was analyzed by FT-Raman. μEDXRF examined calcium (Ca) and phosphorus (P) content as well as Ca/P ratio. Impressions of specimens were evaluated by SEM. Data were submitted to Kruskal-Wallis and Dunn tests (p<0.05). Differences were observed among groups for the 960 cm-1 peak. Ca and P content differences were significant (SH5>NT = SH5E>CHX>E>CHXE). CHXE and E presented the highest Ca/P ratio values compared to the other groups (p<0.05). The SEM images in the EDTA-treated groups had the highest number of open tubules. Erosion in the tubules was observed in CHX and SH5E groups. Endodontic agents change the inorganic and organic content of pulp chamber dentin. NaOCl used alone, or in association with EDTA, was the most effective agent considering chemical and morphological approaches.

Paper Details

Date Published: 9 July 2012
PDF: 7 pages
J. Biomed. Opt. 17(7) 075008 doi: 10.1117/1.JBO.17.7.075008
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Fernanda Miori Pascon, Faculdade de Odontologia de Piracicaba (Brazil)
Regina Maria Puppin-Rontani, Faculdade de Odontologia de Piracicaba (Brazil)
Kamila R. Kantovitz, National Institutes of Health (United States)
Luis E. Soares, Univ. do Vale do Paraíba (Brazil)
Ana M. do Espirito Santo, Univ. Federal de São Paulo (Brazil)
Airton A. Martin, Univ. do Vale do Paraíba (Brazil)

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