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Journal of Biomedical Optics

Three-dimensional simultaneous optical coherence tomography and confocal fluorescence microscopy for investigation of lung tissue
Author(s): Maria Gärtner; Peter Cimalla; Sven Meissner; Edmund Koch; Wolfgang Michael Kübler
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Paper Abstract

Although several strategies exist for a minimal-invasive treatment of patients with lung failure, the mortality rate of acute respiratory distress syndrome still reaches 30% at minimum. This striking number indicates the necessity of understanding lung dynamics on an alveolar level. To investigate the dynamical behavior on a microscale, we used three-dimensional geometrical and functional imaging to observe tissue parameters including alveolar size and length of embedded elastic fibers during ventilation. We established a combined optical coherence tomography (OCT) and confocal fluorescence microscopy system that is able to monitor the distension of alveolar tissue and elastin fibers simultaneously within three dimensions. The OCT system can laterally resolve a 4.9 μm line pair feature and has an approximately 11 μm full-width-half-maximum axial resolution in air. confocal fluorescence microscopy visualizes molecular properties of the tissue with a resolution of 0.75 μm (laterally), and 5.9 μm (axially) via fluorescence detection of the dye sulforhodamine B specifically binding to elastin. For system evaluation, we used a mouse model in situ to perform lung distension by application of different constant pressure values within the physiological regime. Our method enables the investigation of alveolar dynamics by helping to reveal basic processes emerging during artificial ventilation and breathing.

Paper Details

Date Published: 14 June 2012
PDF: 9 pages
J. Biomed. Opt. 17(7) 071310 doi: 10.1117/1.JBO.17.7.071310
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Maria Gärtner, Universitätsklinikum Carl Gustav Carus Dresden (Germany)
Peter Cimalla, Universitätsklinikum Carl Gustav Carus Dresden (Germany)
Sven Meissner, Universitätsklinikum Carl Gustav Carus Dresden (Germany)
Edmund Koch, Universitätsklinikum Carl Gustav Carus Dresden (Germany)
Wolfgang Michael Kübler, Charité Universitätsmedizin Berlin (Germany)

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