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Journal of Biomedical Optics

Imaging a photodynamic therapy photosensitizer in vivo with a time-gated fluorescence tomography system
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Paper Abstract

We report the tomographic imaging of a photodynamic therapy (PDT) photosensitizer, 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a (HPPH) in vivo with time-domain fluorescence diffuse optical tomography (TD-FDOT). Simultaneous reconstruction of fluorescence yield and lifetime of HPPH was performed before and after PDT. The methodology was validated in phantom experiments, and depth-resolved in vivo imaging was achieved through simultaneous three-dimensional (3-D) mappings of fluorescence yield and lifetime contrasts. The tomographic images of a human head-and-neck xenograft in a mouse confirmed the preferential uptake and retention of HPPH by the tumor 24-h post-injection. HPPH-mediated PDT induced significant changes in fluorescence yield and lifetime. This pilot study demonstrates that TD-FDOT may be a good imaging modality for assessing photosensitizer distributions in deep tissue during PDT monitoring.

Paper Details

Date Published: 16 May 2012
PDF: 9 pages
J. Biomed. Opt. 17(7) 071306 doi: 10.1117/1.JBO.17.7.071306
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Weirong Mo, Roswell Park Cancer Institute (United States)
Daniel J. Rohrbach, Roswell Park Cancer Institute (United States)
Ulas Sunar, Roswell Park Cancer Institute (United States)

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