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Journal of Biomedical Optics • Open Access

Label-free subcellular 3D live imaging of preimplantation mouse embryos with full-field optical coherence tomography
Author(s): Jing-gao Zheng; Tianyuan Chen; Chengming Wang; Ning Tian; Fengying Zhao; Tiancheng Huo; Ning Zhang; Dieyan Chen; Wanyun Ma; Jia-Lin Sun; Ping Xue; Danyu Lu

Paper Abstract

Early patterning and polarity is of fundamental interest in preimplantation embryonic development. Label-free subcellular 3D live imaging is very helpful to its related studies. We have developed a novel system of full-field optical coherence tomography (FF-OCT) for noninvasive 3D subcellular live imaging of preimplantation mouse embryos with no need of dye labeling. 3D digitized embryos can be obtained by image processing. Label-free 3D live imaging is demonstrated for the mouse embryos at various typical preimplantation stages with a spatial resolution of 0.7 µm and imaging rate of 24 fps. Factors that relate to early patterning and polarity, such as pronuclei in zygote, shapes of zona pellucida, location of second polar body, cleavage planes, and the blastocyst axis, can be quantitatively measured. The angle between the two second cleavage planes is accurately measured to be 87 deg. It is shown that FF-OCT provides a potential breakthrough for early patterning, polarity formation, and many other preimplantation-related studies in mammalian developmental biology.

Paper Details

Date Published: 28 June 2012
PDF: 4 pages
J. Biomed. Opt. 17(7) 070503 doi: 10.1117/1.JBO.17.7.070503
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Jing-gao Zheng, Tsinghua Univ. (China)
Tianyuan Chen, Tsinghua Univ. (China)
Chengming Wang, Tsinghua Univ. (China)
Ning Tian, Tsinghua Univ. (China)
Fengying Zhao, Tsinghua Univ. (China)
Tiancheng Huo, Tsinghua Univ. (China)
Ning Zhang, Tsinghua Univ. (China)
Dieyan Chen, Tsinghua Univ. (China)
Wanyun Ma, Tsinghua Univ. (China)
Jia-Lin Sun, Tsinghua Univ. (China)
Ping Xue, Tsinghua Univ. (China)
Danyu Lu, Peking Univ. (China)


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