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Journal of Biomedical Optics • Open Access

Volumetric quantification of in vitro sonothrombolysis with microbubbles using high-resolution optical coherence tomography
Author(s): Jong S. Kim; Jonathan E. Leeman; Francois T. H. Yu; Xucai Chen; John J. Pacella; Flordeliza S. Villanueva; Kang Kim; Larry Kagemann; Joel S. Schuman

Paper Abstract

Several in vitro and in vivo studies have established accelerated thrombolysis using ultrasound (US) induced microbubble (MB) cavitation. However, the mechanisms underlying MB mediated sonothrombolysis are still not completely elucidated. We performed three-dimensional (3-D) volumetric optical coherence tomography (OCT) imaging before and after the application of contrast US to thrombus. The most dramatic reduction in clot volume was observed with US + MB + recombinant tissue plasminogen activator (rt-PA). Thrombus surface erosion in this group on the side of the thrombus exposed to MB and ultrasound was evident on the OCT images. This technique may assist in clarifying the mechanisms underlying sonothrombolysis, especially regarding the importance of US transducer orientation on lytic efficacy and the effects of MB cavitation on thrombus structure.

Paper Details

Date Published: 28 June 2012
PDF: 4 pages
J. Biomed. Opt. 17(7) 070502 doi: 10.1117/1.JBO.17.7.070502
Published in: Journal of Biomedical Optics Volume 17, Issue 7
Show Author Affiliations
Jong S. Kim, Univ. of Pittsburgh Medical Ctr. (United States)
Jonathan E. Leeman, Univ. of Pittsburgh Medical Ctr. (United States)
Francois T. H. Yu, Univ. of Pittsburgh Medical Ctr. (United States)
Xucai Chen, Univ. of Pittsburgh Medical Ctr. (United States)
John J. Pacella, Univ. of Pittsburgh Medical Ctr. (United States)
Flordeliza S. Villanueva, Univ. of Pittsburgh Medical Ctr. (United States)
Kang Kim, Univ. of Pittsburgh Medical Ctr. (United States)
Larry Kagemann, Univ. of Pittsburgh Medical Ctr. (United States)
Joel S. Schuman, Univ. of Pittsburgh Medical Ctr. (United States)


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