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Journal of Biomedical Optics • Open Access

Laser-induced modifications of gold nanoparticles and their cytotoxic effect
Author(s): Shimaa Abdelhamid; Hazem Saleh; Mahmoud Abdelhamid; Tareq Youssef; Adel Gohar

Paper Abstract

As nanotechnology continues to develop, an assessment of nanoparticles' toxicity becomes very crucial for biomedical applications. The current study examines the deleterious effects of pre-irradiated gold nanoparticles (GNPs) solutions on primary rat kidney cells (PRKCs). Spectroscopic and transmission electron microscopic studies demonstrated that exposure of 15 nm GNPs in size to pulsed laser caused a reduction both in optical density and mean particle diameter. GNPs showed an aggregation when added to the cell culture medium (DMEM). This aggregation was markedly decreased upon adding serum to the medium. Under our experimental conditions, trypan blue and MTT assays revealed no significant changes in cell viability when PRKCs were incubated with non-irradiated GNPs over a period of 72 h and up to 4 nM GNPs concentration. On the contrary, when cells were incubated with irradiated GNPs a significant reduction in PRKCs viability was revealed.

Paper Details

Date Published: 5 June 2012
PDF: 8 pages
J. Biomed. Opt. 17(6) 068001 doi: 10.1117/1.JBO.17.6.068001
Published in: Journal of Biomedical Optics Volume 17, Issue 6
Show Author Affiliations
Shimaa Abdelhamid, Cairo Univ. (Egypt)
Hazem Saleh, Cairo Univ. (Egypt)
Mahmoud Abdelhamid, Cairo Univ. (Egypt)
Tareq Youssef, Cairo Univ. (Egypt)
Adel Gohar, Cairo Univ. (Egypt)


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