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Journal of Biomedical Optics

Label-free serum ribonucleic acid analysis for colorectal cancer detection by surface-enhanced Raman spectroscopy and multivariate analysis
Author(s): Yanping Chen; Shangyuan Feng; Zufang Huang; Xiaoqian Lin; Rong Chen; Gang Chen; Jianji Pan; Xiongwei Zheng; Ying Su; Yan Chen; Fenghua Lan; Haishan Zeng
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Paper Abstract

Studies with circulating ribonucleic acid (RNA) not only provide new targets for cancer detection, but also open up the possibility of noninvasive gene expression profiling for cancer. In this paper, we developed a surface-enhanced Raman scattering (SERS), platform for detection and differentiation of serum RNAs of colorectal cancer. A novel three-dimensional (3-D), Ag nanofilm formed by dry MgSO4 aggregated silver nanoparticles, Ag NP, as the SERS-active substrate was presented to effectively enhance the RNA Raman signals. SERS measurements were performed on two groups of serum RNA samples. One group from patients, n = 55 with pathologically diagnosed colorectal cancer and the other group from healthy controls, n = 45. Tentative assignments of the Raman bands in the normalized SERS spectra demonstrated that there are differential expressions of cancer-related RNAs between the two groups. Linear discriminate analysis, based on principal component analysis, generated features can differentiate the colorectal cancer SERS spectra from normal SERS spectra with sensitivity of 89.1 percent and specificity of 95.6 percent. This exploratory study demonstrated great potential for developing serum RNA SERS analysis into a useful clinical tool for label-free, noninvasive screening and detection of colorectal cancers.

Paper Details

Date Published: 6 June 2012
PDF: 8 pages
J. Biomed. Opt. 17(6) 067003 doi: 10.1117/1.JBO.17.6.067003
Published in: Journal of Biomedical Optics Volume 17, Issue 6
Show Author Affiliations
Yanping Chen, Fujian Normal Univ. (China)
Shangyuan Feng, Fujian Normal Univ. (China)
Zufang Huang, Fujian Normal Univ. (China)
Xiaoqian Lin, Fujian Normal Univ. (China)
Rong Chen, Fujian Normal Univ. (China)
Gang Chen, Fujian Medical Univ. (China)
Jianji Pan, Fujian Medical Univ. (China)
Xiongwei Zheng, Fujian Medical Univ. (China)
Ying Su, Fujian Medical Univ. (China)
Yan Chen, Fujian Medical Univ. (China)
Fenghua Lan, Fuzhou General Hospital (China)
Haishan Zeng, The BC Cancer Agency Research Ctr. (Canada)

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