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Journal of Biomedical Optics • Open Access

In vivo quantification of photosensitizer concentration using fluorescence differential path-length spectroscopy: influence of photosensitizer formulation and tissue location

Paper Abstract

In vivo measurement of photosensitizer concentrations may optimize clinical photodynamic therapy (PDT). Fluorescence differential path-length spectroscopy (FDPS) is a non-invasive optical technique that has been shown to accurately quantify the concentration of Foscan® in rat liver. As a next step towards clinical translation, the effect of two liposomal formulations of mTHPC, Fospeg® and Foslip®, on FDPS response was investigated. Furthermore, FDPS was evaluated in target organs for head-and-neck PDT. Fifty-four healthy rats were intravenously injected with one of the three formulations of mTHPC at 0.15  mg kg−1. FDPS was performed on liver, tongue, and lip. The mTHPC concentrations estimated using FDPS were correlated with the results of the subsequent harvested and chemically extracted organs. An excellent goodness of fit (R2) between FDPS and extraction was found for all formulations in the liver (R2 = 0.79). A much lower R2 between FDPS and extraction was found in lip (R2 = 0.46) and tongue (R2 = 0.10). The lower performance in lip and in particular tongue was mainly attributed to the more layered anatomical structure, which influences scattering properties and photosensitizer distribution.

Paper Details

Date Published: 4 June 2012
PDF: 10 pages
J. Biomed. Opt. 17(6) 067001 doi: 10.1117/1.JBO.17.6.067001
Published in: Journal of Biomedical Optics Volume 17, Issue 6
Show Author Affiliations
Sebastiaan A. de Visscher, Univ. Medical Ctr. Groningen (Netherlands)
Max J. Witjes, Univ. Medical Ctr. Groningen (Netherlands)
Jan L. N. Roodenburg, Univ. Medical Ctr. Groningen (Netherlands)
Arjen Amelink, Univ. Medical Ctr. Groningen (Netherlands)
Slávka Kascáková, Erasmus MC (Netherlands)
Henricus J. Sterenborg, Erasmus MC (Netherlands)
Dominic J. Robinson, Erasmus MC (Netherlands)


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