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Journal of Biomedical Optics • Open Access

Monitoring the process of pulmonary melanoma metastasis using large area and label-free nonlinear optical microscopy
Author(s): Daozhu Hua; Shuhong Qi; Hui Li; Zhihong Zhang; Ling Fu

Paper Abstract

We performed large area nonlinear optical microscopy (NOM) for label-free monitoring of the process of pulmonary melanoma metastasis ex vivo with subcellular resolution in C57BL/6 mice. Multiphoton autofluorescence (MAF) and second harmonic generation (SHG) images of lung tissue are obtained in a volume of ∼ 2.2  mm×2.2  mm×30  μm. Qualitative differences in morphologic features and quantitative measurement of pathological lung tissues at different time points are characterized. We find that combined with morphological features, the quantitative parameters, such as the intensity ratio of MAF and SHG between pathological tissue and normal tissue and the MAF to SHG index versus depth clearly shows the tissue physiological changes during the process of pulmonary melanoma metastasis. Our results demonstrate that large area NOM succeeds in monitoring the process of pulmonary melanoma metastasis, which can provide a powerful tool for the research in tumor pathophysiology and therapy evaluation.

Paper Details

Date Published: 5 June 2012
PDF: 7 pages
J. Biomed. Opt. 17(6) 066002 doi: 10.1117/1.JBO.17.6.066002
Published in: Journal of Biomedical Optics Volume 17, Issue 6
Show Author Affiliations
Daozhu Hua, Huazhong Univ. of Science and Technology (China)
Shuhong Qi, Huazhong Univ. of Science and Technology (China)
Hui Li, Huazhong Univ. of Science and Technology (China)
Zhihong Zhang, Britton Chance Ctr. for Biomedical Photonics (China)
Ling Fu, Britton Chance Ctr. for Biomedical Photonics (China)


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