Share Email Print

Journal of Biomedical Optics • Open Access

Improved tumor contrast achieved by single time point dual-reporter fluorescence imaging

Paper Abstract

In this study, we demonstrate a method to quantify biomarker expression that uses an exogenous dual-reporter imaging approach to improve tumor signal detection. The uptake of two fluorophores, one nonspecific and one targeted to the epidermal growth factor receptor (EGFR), were imaged at 1 h in three types of xenograft tumors spanning a range of EGFR expression levels (n = 6 in each group). Using this dual-reporter imaging methodology, tumor contrast-to-noise ratio was amplified by <6 times at 1 h postinjection and <2 times at 24 h. Furthermore, by as early as 20 min postinjection, the dual-reporter imaging signal in the tumor correlated significantly with a validated marker of receptor density (P<0.05, r = 0.93). Dual-reporter imaging can improve sensitivity and specificity over conventional fluorescence imaging in applications such as fluorescence-guided surgery and directly approximates the receptor status of the tumor, a measure that could be used to inform choices of biological therapies.

Paper Details

Date Published: 5 June 2012
PDF: 11 pages
J. Biomed. Opt. 17(6) 066001 doi: 10.1117/1.JBO.17.6.066001
Published in: Journal of Biomedical Optics Volume 17, Issue 6
Show Author Affiliations
Kenneth M. Tichauer, Thayer School of Engineering at Dartmouth (United States)
Kimberley S. Samkoe, Dartmouth Hitchcock Medical Ctr. (United States)
Kristian J. Sexton, Thayer School of Engineering at Dartmouth (United States)
Jason R. Gunn, Thayer School of Engineering at Dartmouth (United States)
Tayyaba Hasan, Massachusetts General Hospital (United States)
Brian W. Pogue, Thayer School of Engineering at Dartmouth (United States)

© SPIE. Terms of Use
Back to Top