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Journal of Biomedical Optics

Capture of circulating tumor cells using photoacoustic flowmetry and two phase flow
Author(s): Christine M. O'Brien; Kyle Rood; Kiran D. Bhattacharyya; Thiago DeSouza; Shramik Sengupta; Sagar K. Gupta; Jeff Mosley; Benjamin S. Goldschmidt; John A. Viator; Nikhilesh Sharma
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Paper Abstract

Melanoma is the deadliest form of skin cancer, yet current diagnostic methods are unable to detect early onset of metastatic disease. Patients must wait until macroscopic secondary tumors form before malignancy can be diagnosed and treatment prescribed. Detection of cells that have broken off the original tumor and travel through the blood or lymph system can provide data for diagnosing and monitoring metastatic disease. By irradiating enriched blood samples spiked with cultured melanoma cells with nanosecond duration laser light, we induced photoacoustic responses in the pigmented cells. Thus, we can detect and enumerate melanoma cells in blood samples to demonstrate a paradigm for a photoacoustic flow cytometer. Furthermore, we capture the melanoma cells using microfluidic two phase flow, a technique that separates a continuous flow into alternating microslugs of air and blood cell suspension. Each slug of blood cells is tested for the presence of melanoma. Slugs that are positive for melanoma, indicated by photoacoustic waves, are separated from the cytometer for further purification and isolation of the melanoma cell. In this paper, we evaluate the two phase photoacoustic flow cytometer for its ability to detect and capture metastastic melanoma cells in blood.

Paper Details

Date Published: 7 May 2012
PDF: 10 pages
J. Biomed. Opt. 17(6) 061221 doi: 10.1117/1.JBO.17.6.061221
Published in: Journal of Biomedical Optics Volume 17, Issue 6
Show Author Affiliations
Christine M. O'Brien, Univ. of Missouri-Columbia (United States)
Kyle Rood, Univ. of Missouri-Columbia (United States)
Kiran D. Bhattacharyya, Univ. of Missouri-Columbia (United States)
Thiago DeSouza, Univ. of Missouri-Columbia (United States)
Shramik Sengupta, Univ. of Missouri-Columbia (United States)
Sagar K. Gupta, Univ. of Missouri-Columbia (United States)
Jeff Mosley, Univ. of Missouri-Columbia (United States)
Benjamin S. Goldschmidt, Univ. of Missouri-Columbia (United States)
John A. Viator, Univ. of Missouri-Columbia (United States)
Nikhilesh Sharma, Univ. of Missouri-Columbia (United States)

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