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Journal of Biomedical Optics • Open Access

Label-free photoacoustic microscopy of myocardial sheet architecture
Author(s): Chi Zhang; Lihong V. Wang; Ya-Jian Cheng; Junjie Chen; Samuel A. Wickline

Paper Abstract

Cardiac myofibers are organized into sheet architectures, which contribute to up to 40% of the heart wall thickening for ejection of blood for circulation. It is important to delineate the sheet architecture for a better understanding of cardiac mechanisms. However, current sheet imaging technologies are limited by fixation-induced dehydration/deformation and low spatial resolution. Here we implemented high-resolution label-free photoacoustic microscopy (PAM) of the myocardial sheet architecture. With high endogenous optical-absorption contrast originating mainly from cytochrome, myoglobin, and melanin, PAM can image the unfixed, unstained and unsliced heart without introducing deformation artifacts. A fresh blood-free mouse heart was imaged by PAM ex vivo. The three-dimensional branching sheets were clearly identified within 150 µm depth. Various morphological parameters were derived from the PAM image. The sheet thickness (80±10 μm) and the cleavage height (11±1 μm) were derived from an undehydrated heart for the first time. Therefore, PAM has the potential for the functional imaging of sheet architecture in ex vivo perfused and viable hearts.

Paper Details

Date Published: 6 June 2012
PDF: 4 pages
J. Biomed. Opt. 17(6) 060506 doi: 10.1117/1.JBO.17.6.060506
Published in: Journal of Biomedical Optics Volume 17, Issue 6
Show Author Affiliations
Chi Zhang, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)
Ya-Jian Cheng, Washington Univ. School of Medicine in St. Louis (United States)
Junjie Chen, Washington Univ. School of Medicine in St. Louis (United States)
Samuel A. Wickline, Washington Univ. School of Medicine in St. Louis (United States)

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