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Journal of Biomedical Optics • Open Access

Investigation of the hyper-reflective inner/outer segment band in optical coherence tomography of living frog retina
Author(s): Rong-Wen Lu; Qiu-Xiang Zhang; Xincheng Yao; Christine A. Curcio; Youwen Zhang; Steven Pittler; Dusanka Deretic

Paper Abstract

This study is to test anatomic correlates, including connecting cilium (CC) and inner segment (IS) ellipsoid, to the hyper-reflective band visualized by optical coherence tomography (OCT) and commonly attributed to the photoreceptor inner/outer segment (IS/OS) junction. A line-scan OCT (LS-OCT) was constructed to achieve sub-cellular resolution (lateral: ~ 2 μm; axial: ~ 4 μm) of excised living frog retinas. An electro-optic phase modulator was employed for rapid and vibration-free phase modulation. Comparison of normalized distance measurements between LS-OCT images and histological images revealed that the dominant source of the signal reported as the IS/OS OCT band actually originates from the IS.

Paper Details

Date Published: 6 June 2012
PDF: 4 pages
J. Biomed. Opt. 17(6) 060504 doi: 10.1117/1.JBO.17.6.060504
Published in: Journal of Biomedical Optics Volume 17, Issue 6
Show Author Affiliations
Rong-Wen Lu, The Univ. of Alabama at Birmingham (United States)
Qiu-Xiang Zhang, The Univ. of Alabama at Birmingham (United States)
Xincheng Yao, The Univ. of Alabama at Birmingham (United States)
Christine A. Curcio, The Univ. of Alabama at Birmingham (United States)
Youwen Zhang, The Univ. of Alabama at Birmingham (United States)
Steven Pittler, The Univ. of Alabama at Birmingham (United States)
Dusanka Deretic, The Univ. of New Mexico (United States)


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