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Journal of Biomedical Optics • Open Access

Functional photoacoustic microscopy of diabetic vasculature
Author(s): Arie Krumholz; Lidai Wang; Junjie Yao; Lihong V. Wang

Paper Abstract

We used functional photoacoustic microscopy to image diabetes-induced damage to the microvasculature. To produce an animal model for Type 1 diabetes, we used streptozotocin (STZ), which is particularly toxic to the insulin-producing beta cells of the pancreas in mammals. A set number of ND4 Swiss Webster mice received intraperitoneal injections of STZ for five consecutive days at 50 mg/kg. Most mice developed a significant rise in blood glucose level ( ∼ 400 mg/dL) within three weeks of the first injection. Changes in vasculature and hemodynamics were monitored for six weeks. The mouse ear was imaged with an optical-resolution photoacoustic microscope at a main blood vessel branch from the root of the ear. There are noticeable and measurable changes associated with the disease, including decreased vessel diameter and possible occlusion due to vessel damage and polyurea. We also observed an increase in the blood flow speed in the vein and a decrease in the artery, which could be due to compensation for the dehydration and vessel diameter changes. Functional and metabolic parameters such as hemoglobin oxygen saturation, oxygen extraction fraction, and oxygen consumption rate were also measured, but showed no significant change.

Paper Details

Date Published: 21 May 2012
PDF: 4 pages
J. Biomed. Opt. 17(6) 060502 doi: 10.1117/1.JBO.17.6.060502
Published in: Journal of Biomedical Optics Volume 17, Issue 6
Show Author Affiliations
Arie Krumholz, Washington Univ. in St. Louis (United States)
Lidai Wang, Washington Univ. in St. Louis (United States)
Junjie Yao, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)

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