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Journal of Biomedical Optics

Photochemical internalization of bleomycin for glioma treatment
Author(s): Marlon S. Mathews; En-Chung Shih; Genesis Zamora; Chung-Ho Sun; Henry Hirschberg; Joseph Blickenstaff; Van Vo; Steen J. Madsen
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Paper Abstract

We study the use of photochemical internalization (PCI) for enhancing chemotherapeutic response to malignant glioma cells in vitro. Two models are studied: monolayers consisting of F98 rat glioma cells and human glioma spheroids established from biopsy-derived glioma cells. In both cases, the cytotoxicity of aluminum phthalocyanine disulfonate (AlPcS2a)-based PCI of bleomycin was compared to AlPcS2a-photodynamic therapy (PDT) and chemotherapy alone. Monolayers and spheroids were incubated with AlPcS2a (PDT effect), bleomycin (chemotherapy effect), or AlPcS2a+bleomycin (PCI effect) and were illuminated (670 nm). Toxicity was evaluated using colony formation assays or spheroid growth kinetics. F98 cells in monolayer/spheroids were not particularly sensitive to the effects of low radiant exposure (1.5  J/cm2 @ 5  mW/cm2) AlPcS2a-PDT. Bleomycin was moderately toxic to F98 cells in monolayer at relatively low concentrations-incubation of F98 cells in 0.1  μg/ml for 4 h resulted in 80% survival, but less toxic in human glioma spheroids respectively. In both in vitro systems investigated, a significant PCI effect is seen. PCI using 1.5  J/cm2 together with 0.25  μg/ml bleomycin resulted in approximately 20% and 18% survival of F98 rat glioma cells and human glioma spheroids, respectively. These results show that AlPcS2a-mediated PCI can be used to enhance the efficacy of chemotherapeutic agents such as bleomycin in malignant gliomas.

Paper Details

Date Published: 3 May 2012
PDF: 9 pages
J. Biomed. Opt. 17(5) 058001 doi: 10.1117/1.JBO.17.5.058001
Published in: Journal of Biomedical Optics Volume 17, Issue 5
Show Author Affiliations
Marlon S. Mathews, Univ. of California, Irvine (United States)
En-Chung Shih, Beckman Laser Institute and Medical Clinic (United States)
Genesis Zamora, Beckman Laser Institute and Medical Clinic (United States)
Chung-Ho Sun, Beckman Laser Institute and Medical Clinic (United States)
Henry Hirschberg, Beckman Laser Institute and Medical Clinic (United States)
Joseph Blickenstaff, Univ. of Nevada, Las Vegas (United States)
Van Vo, Univ. of Nevada, Las Vegas (United States)
Steen J. Madsen, Univ. of Nevada, Las Vegas (United States)


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