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Journal of Biomedical Optics

Noninvasive in vivo optical assessment of blood brain barrier permeability and brain tissue drug deposition in rabbits
Author(s): Aysegul Ergin; Jane Y. Zhang; Mei Wang; Shailendra Joshi; Irving J. Bigio
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Paper Abstract

Osmotic disruption of the blood brain barrier (BBB) by intraarterial mannitol injection is sometimes the key step for the delivery of chemotherapeutic drugs to brain tissue. BBB disruption (BBBD) with mannitol, however, can be highly variable and could impact local drug deposition. We use optical pharmacokinetics, which is based on diffuse reflectance spectroscopy, to track in vivo brain tissue concentrations of indocyanine green (ICG), an optical reporter used to monitor BBBD, and mitoxantrone (MTX), a chemotherapy agent that does not deposit in brain tissue without BBBD, in anesthetized New Zealand white rabbits. Results show a significant increase in the tissue ICG concentrations with BBBD, and our method is able to track the animal-to-animal variation in tissue ICG and MTX concentrations after BBBD. The tissue concentrations of MTX increase with barrier disruption and are found to be correlated to the degree of disruption, as assessed by the ICG prior to the injection of the drug. These findings should encourage the development of tracers and optical methods capable of quantifying the degree of BBBD, with the goal of improving drug delivery.

Paper Details

Date Published: 21 May 2012
PDF: 11 pages
J. Biomed. Opt. 17(5) 057008 doi: 10.1117/1.JBO.17.5.057008
Published in: Journal of Biomedical Optics Volume 17, Issue 5
Show Author Affiliations
Aysegul Ergin, Boston Univ. (United States)
Jane Y. Zhang, Boston Univ. (United States)
Mei Wang, Columbia Univ. (United States)
Shailendra Joshi, Columbia Univ. (United States)
Irving J. Bigio, Boston Univ. (United States)

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