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Journal of Biomedical Optics

Light-scattering flow cytometry for identification and characterization of blood microparticles
Author(s): Anastasiya I. Konokhova; Maxim A. Yurkin; Alexander E. Moskalensky; Andrei V. Chernyshev; Valeri P. Maltsev; Elena D. Chikova; Galina A. Tsvetovskaya
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Paper Abstract

We describe a novel approach to study blood microparticles using the scanning flow cytometer, which measures light scattering patterns (LSPs) of individual particles. Starting from platelet-rich plasma, we separated spherical microparticles from non-spherical plasma constituents, such as platelets and cell debris, based on similarity of their LSP to that of sphere. This provides a label-free method for identification (detection) of microparticles, including those larger than 1 µm. Next, we rigorously characterized each measured particle, determining its size and refractive index including errors of these estimates. Finally, we employed a deconvolution algorithm to determine size and refractive index distributions of the whole population of microparticles, accounting for largely different reliability of individual measurements. Developed methods were tested on a blood sample of a healthy donor, resulting in good agreement with literature data. The only limitation of this approach is size detection limit, which is currently about 0.5 µm due to used laser wavelength of 0.66 µm.

Paper Details

Date Published: 10 May 2012
PDF: 9 pages
J. Biomed. Opt. 17(5) 057006 doi: 10.1117/1.JBO.17.5.057006
Published in: Journal of Biomedical Optics Volume 17, Issue 5
Show Author Affiliations
Anastasiya I. Konokhova, Institute of Chemical Kinetics and Combustion (Russian Federation)
Maxim A. Yurkin, Institute of Chemical Kinetics and Combustion (Russian Federation)
Alexander E. Moskalensky, Institute of Chemical Kinetics and Combustion (Russian Federation)
Andrei V. Chernyshev, Institute of Chemical Kinetics and Combustion (Russian Federation)
Valeri P. Maltsev, Institute of Chemical Kinetics and Combustion (Russian Federation)
Elena D. Chikova, Novosibirsk State Univ. (Russian Federation)
Galina A. Tsvetovskaya, Ctr. for New Medical Technologies (TEMPO) (Russian Federation)

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