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Journal of Biomedical Optics • Open Access

Digital simulation of staining in histopathology multispectral images: enhancement and linear transformation of spectral transmittance

Paper Abstract

Hematoxylin and eosin (H&E) stain is currently the most popular for routine histopathology staining. Special and/or immuno-histochemical (IHC) staining is often requested to further corroborate the initial diagnosis on H&E stained tissue sections. Digital simulation of staining (or digital staining) can be a very valuable tool to produce the desired stained images from the H&E stained tissue sections instantaneously. We present an approach to digital staining of histopathology multispectral images by combining the effects of spectral enhancement and spectral transformation. Spectral enhancement is accomplished by shifting the N-band original spectrum of the multispectral pixel with the weighted difference between the pixel's original and estimated spectrum; the spectrum is estimated using M < N principal component (PC) vectors. The pixel's enhanced spectrum is transformed to the spectral configuration associated to its reaction to a specific stain by utilizing an N × N transformation matrix, which is derived through application of least mean squares method to the enhanced and target spectral transmittance samples of the different tissue components found in the image. Results of our experiments on the digital conversion of an H&E stained multispectral image to its Masson's trichrome stained equivalent show the viability of the method.

Paper Details

Date Published: 4 May 2012
PDF: 11 pages
J. Biomed. Opt. 17(5) 056013 doi: 10.1117/1.JBO.17.5.056013
Published in: Journal of Biomedical Optics Volume 17, Issue 5
Show Author Affiliations
Pinky A. Bautista, Massachusetts General Hospital (United States)
Yukako Yagi, Massachusetts General Hospital (United States)


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