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Journal of Biomedical Optics • Open Access

Spatial frequency domain tomography of protoporphyrin IX fluorescence in preclinical glioma models

Paper Abstract

Multifrequency (0 to 0.3  mm−1), multiwavelength (633, 680, 720, 800, and 820 nm) spatial frequency domain imaging (SFDI) of 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) was used to recover absorption, scattering, and fluorescence properties of glioblastoma multiforme spheroids in tissue-simulating phantoms and in vivo in a mouse model. Three-dimensional tomographic reconstructions of the frequency-dependent remitted light localized the depths of the spheroids within 500 μm, and the total amount of PpIX in the reconstructed images was constant to within 30% when spheroid depth was varied. In vivo tumor-to-normal contrast was greater than ∼ 1.5 in reduced scattering coefficient for all wavelengths and was ∼ 1.3 for the tissue concentration of deoxyhemoglobin (ctHb). The study demonstrates the feasibility of SFDI for providing enhanced image guidance during surgical resection of brain tumors.

Paper Details

Date Published: 4 May 2012
PDF: 8 pages
J. Biomed. Opt. 17(5) 056008 doi: 10.1117/1.JBO.17.5.056008
Published in: Journal of Biomedical Optics Volume 17, Issue 5
Show Author Affiliations
Soren D. Konecky, Beckman Laser Institute and Medical Clinic (United States)
Tyler B. Rice, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)
Chris M.. Owen, Univ. of California, Irvine (United States)
Pablo A. Valdes, Dartmouth Hitchcock Medical Ctr. (United States)
David W. Roberts, Dartmouth Hitchcock Medical Ctr. (United States)
Kolbein Kolste, Thayer School of Engineering at Dartmouth (United States)
Frederic Leblond, Thayer School of Engineering at Dartmouth (United States)
Keith D. Paulsen, Thayer School of Engineering at Dartmouth (United States)
Brian C. Wilson, Ontario Cancer Institute (Canada)


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