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Journal of Biomedical Optics

Optimal ultraviolet wavelength for in vivo photoacoustic imaging of cell nuclei
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Paper Abstract

In order to image noninvasively cell nuclei in vivo without staining, we have developed ultraviolet photoacoustic microscopy (UV-PAM), in which ultraviolet light excites nucleic acids in cell nuclei to produce photoacoustic waves. Equipped with a tunable laser system, the UV-PAM was applied to in vivo imaging of cell nuclei in small animals. We found that 250 nm was the optimal wavelength for in vivo photoacoustic imaging of cell nuclei. The optimal wavelength enables UV-PAM to image cell nuclei using as little as 2 nJ laser pulse energy. Besides the optimal wavelength, application of a wavelength between 245 and 275 nm can produce in vivo images of cell nuclei with specific, positive, and high optical contrast.

Paper Details

Date Published: 4 May 2012
PDF: 8 pages
J. Biomed. Opt. 17(5) 056004 doi: 10.1117/1.JBO.17.5.056004
Published in: Journal of Biomedical Optics Volume 17, Issue 5
Show Author Affiliations
Da-Kang Yao, Washington Univ. in St. Louis (United States)
Konstantin I. Maslov, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)
Ruimin Chen, The Univ. of Southern California (United States)
Qifa Zhou, The Univ. of Southern California (United States)


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