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Journal of Biomedical Optics

Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics
Author(s): Martí Duocastella; Bo Sun; Craig B. Arnold
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Paper Abstract

Traditional white-light and fluorescent imaging techniques provide powerful methods to extract high-resolution information from two-dimensional (2-D) sections, but to retrieve information from a three-dimensional (3-D) volume they require relatively slow scanning methods that result in increased acquisition time. Using an ultra-high speed liquid lens, we circumvent this problem by simultaneously acquiring images from multiple focal planes. We demonstrate this method by imaging microparticles and cells flowing in 3-D microfluidic channels.

Paper Details

Date Published: 7 May 2012
PDF: 4 pages
J. Biomed. Opt. 17(5) 050505 doi: 10.1117/1.JBO.17.5.050505
Published in: Journal of Biomedical Optics Volume 17, Issue 5
Show Author Affiliations
Martí Duocastella, Princeton Univ. (United States)
Bo Sun, Princeton Univ. (United States)
Craig B. Arnold, Princeton Univ. (United States)

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