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Journal of Biomedical Optics • Open Access

otential clinical impact of three-dimensional visualization for fluorescent in situ hybridization image analysis
Author(s): Zheng Li; Yuhua Li; Hong Liu; Shibo Li; Roy Zhang; Huimin Tian; Bin Zheng; Wei R. Chen

Paper Abstract

Chromosomal translocation is strong indication of cancers. Fluorescent in situ hybridization (FISH) can effectively detect this translocation and achieve high accuracy in disease diagnosis and prognosis assessment. For this purpose, whole chromosome paint probes are utilized to image the configuration of DNA fragments. Although two-dimensional (2-D) microscopic images are typically used in FISH signal analysis, we present a case where the translocation occurs in the depth direction where two probed FISH signals are overlapped in the projected image plane. Thus, the translocation cannot be identified. However, when imaging the whole specimen with a confocal microscope at 27 focal planes with 0.5-μm step interval, the translocation can be clearly identified due to the free rotation capability by the three-dimensional (3-D) visualization. Such a translocation detection error of using 2-D images might be critical in detecting and diagnosing early or subtle disease cases where detecting a small number of abnormal cells can make diagnostic difference. Hence, the underlying implication of this report suggests that utilizing 3-D visualization may improve the overall accuracy of FISH analysis for some clinical cases. However, the clinical efficiency and cost of using 3-D versus 2-D imaging methods are also to be assessed carefully.

Paper Details

Date Published: 4 May 2012
PDF: 4 pages
J. Biomed. Opt. 17(5) 050501 doi: 10.1117/1.JBO.17.5.050501
Published in: Journal of Biomedical Optics Volume 17, Issue 5
Show Author Affiliations
Zheng Li, The Univ. of Oklahoma (United States)
Yuhua Li, The Univ. of Oklahoma (United States)
Hong Liu, The Univ. of Oklahoma (United States)
Shibo Li, The Univ. of Oklahoma Health Sciences Ctr. (United States)
Roy Zhang, The Univ. of Oklahoma Health Sciences Ctr. (United States)
Huimin Tian, The Univ. of Oklahoma Health Sciences Ctr. (United States)
Bin Zheng, Univ. of Pittsburgh (United States)
Wei R. Chen, Univ. of Central Oklahoma (United States)

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