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Journal of Biomedical Optics • Open Access

Optical imaging of tissue mitochondrial redox state in intact rat lungs in two models of pulmonary oxidative stress
Author(s): Reyhaneh Sepehr; Kevin Staniszewski; Sepideh Maleki; Mahsa Ranji; Elizabeth R. Jacobs; Said Audi

Paper Abstract

Ventilation with enhanced fractions of O2 (hyperoxia) is a common and necessary treatment for hypoxemia in patients with lung failure, but prolonged exposure to hyperoxia causes lung injury. Ischemia-reperfusion (IR) injury of lung tissue is common in lung transplant or crush injury to the chest. These conditions are associated with apoptosis and decreased survival of lung tissue. The objective of this work is to use cryoimaging to evaluate the effect of exposure to hyperoxia and IR injury on lung tissue mitochondrial redox state in rats. The autofluorescent mitochondrial metabolic coenzymes nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) are electron carriers in ATP generation. These intrinsic fluorophores were imaged for rat lungs using low-temperature fluorescence imaging (cryoimaging). Perfused lungs from four groups of rats were studied: normoxia (control), control perfused with an mitochondrial complex IV inhibitor (potassium cyanide, KCN), rats exposed to hyperoxia (85% O2) for seven days, and from rats subjected to lung IR in vivo 24 hours prior to study. Each lung was sectioned sequentially in the transverse direction, and the images were used to reconstruct a three-dimensional (3-D) rendering. In KCN perfused lungs the respiratory chain was more reduced, whereas hyperoxic and IR lung tissue have a more oxidized respiratory chain than control lung tissue, consistent with previously measured mitochondrial dysfunction in both hyperoxic and IR lungs.

Paper Details

Date Published: 19 April 2012
PDF: 8 pages
J. Biomed. Opt. 17(4) 046010 doi: 10.1117/1.JBO.17.4.046010
Published in: Journal of Biomedical Optics Volume 17, Issue 4
Show Author Affiliations
Reyhaneh Sepehr, Univ. of Wisconsin-Milwaukee (United States)
Kevin Staniszewski, Univ. of Wisconsin-Milwaukee (United States)
Sepideh Maleki, Univ. of Wisconsin-Milwaukee (United States)
Mahsa Ranji, Univ. of Wisconsin-Milwaukee (United States)
Elizabeth R. Jacobs, Medical College of Wisconsin (United States)
Said Audi, Marquette Univ. (United States)

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