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Journal of Biomedical Optics • Open Access

Error analysis of ratiometric imaging of extracellular pH in a window chamber model

Paper Abstract

Ratiometric fluorescence-imaging technique is commonly used to measure extracellular pH in tumors and surrounding tissue within a dorsal skin-fold window chamber. Using a pH-sensitive fluorophore such as carboxy SNARF-1 one can measure pH distributions with high precision. However, it is often observed that the measured pH is lower than expected, with a bias that varies from one image to another. A comprehensive analysis of possible error sources is presented. These error sources include photon noise, estimator bias, instrument errors, temperature, and calibration errors from biological factors.

Paper Details

Date Published: 6 April 2012
PDF: 7 pages
J. Biomed. Opt. 17(4) 046004 doi: 10.1117/1.JBO.17.4.046004
Published in: Journal of Biomedical Optics Volume 17, Issue 4
Show Author Affiliations
Yuxiang Lin, College of Optical Sciences, The Univ. of Arizona (United States)
Tzu-Yu Wu, College of Optical Sciences, The Univ. of Arizona (United States)
Arthur F. Gmitro, The Univ. of Arizona (United States)

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