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Journal of Biomedical Optics • Open Access

Multifunctionality of indocyanine green-loaded biodegradable nanoparticles for enhanced optical imaging and hyperthermia intervention of cancer
Author(s): Ronak H. Patel; Aniket S. Wadajkar; Nimit L. Patel; Venkaiah C. Kavuri; Kytai T. Nguyen; Hanli Liu

Paper Abstract

The aim of this study was to develop and characterize multifunctional biodegradable and biocompatible poly lactic-co-glycolic acid (PLGA) nanoparticles loaded with indocyanine green (ICG) as an optical-imaging contrast agent for cancer imaging and as a photothermal therapy agent for cancer treatment. PLGA-ICG nanoparticles (PIN) were synthesized with a particle diameter of 246±11  nm, a polydispersity index of 0.10±0.03, and ICG loading efficiency of 48.75±5.48%. PIN were optically characterized with peak excitation and emission at 765 and 810± 5  nm, a fluorescence lifetime of 0.30±0.01  ns, and peak absorbance at 780 nm. The cytocompatibility study of PIN showed 85% cell viability till 1-mg/ml concentration of PIN. Successful cellular uptake of ligand conjugated PIN by prostate cancer cells (PC3) was also obtained. Both phantom-based and in vitro cell culture results demonstrated that PIN (1) have the great potential to induce local hyperthermia (i.e., temperature increase of 8 to 10°C) in tissue within 5 mm both in radius and in depth; (2) result in improved optical stability, excellent biocompatibility with healthy cells, and a great targeting capability; (3) have the ability to serve as an image contrast agent for deep-tissue imaging in diffuse optical tomography.

Paper Details

Date Published: 9 April 2012
PDF: 11 pages
J. Biomed. Opt. 17(4) 046003 doi: 10.1117/1.JBO.17.4.046003
Published in: Journal of Biomedical Optics Volume 17, Issue 4
Show Author Affiliations
Ronak H. Patel, The Univ. of Texas at Arlington (United States)
Aniket S. Wadajkar, The Univ. of Texas at Arlington (United States)
Nimit L. Patel, The Univ. of Texas at Arlington (United States)
Venkaiah C. Kavuri, The Univ. of Texas at Arlington (United States)
Kytai T. Nguyen, The Univ. of Texas at Arlington (United States)
Hanli Liu, The Univ. of Texas at Arlington (United States)

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