Share Email Print

Journal of Biomedical Optics • Open Access

In vivo imaging of unstained tissues using a compact and flexible multiphoton microendoscope
Author(s): Christopher M. Brown; David R. Rivera; Dimitre G. Ouzounov; Watt W. Webb; Chris Xu; Ina P. Pavlova; Wendy O. Williams; Sunish Mohanan

Paper Abstract

We use a compact and flexible multiphoton microendoscope (MPME) to acquire in vivo images of unstained liver, kidney, and colon from an anesthetized rat. The device delivers femtosecond pulsed 800 nm light from the core of a raster-scanned dual-clad fiber (DCF), which is focused by a miniaturized gradient-index lens assembly into tissue. Intrinsic fluorescence and second-harmonic generation signal from the tissue is epi-collected through the core and inner clad of the same DCF. The MPME has a rigid distal tip of 3 mm in outer diameter and 4 cm in length. The image field-of-view measures 115 μm by 115 μm and was acquired at 4.1  frames/s with 75 mW illumination power at the sample. Organs were imaged after anesthetizing Sprague-Dawley rats with isofluorane gas, accessing tissues via a ventral-midline abdominal incision, and isolating the organs with tongue depressors. In vivo multiphoton images acquired from liver, kidney, and colon using this device show features similar to that of conventional histology slides, without motion artifact, in ∼ 75% of imaged frames. To the best of our knowledge, this is the first demonstration of multiphoton imaging of unstained tissue from a live subject using a compact and flexible MPME device.

Paper Details

Date Published: 9 April 2012
PDF: 4 pages
J. Biomed. Opt. 17(4) 040505 doi: 10.1117/1.JBO.17.4.040505
Published in: Journal of Biomedical Optics Volume 17, Issue 4
Show Author Affiliations
Christopher M. Brown, Cornell Univ. (United States)
David R. Rivera, Cornell Univ. (United States)
Dimitre G. Ouzounov, Cornell Univ. (United States)
Watt W. Webb, Cornell Univ. (United States)
Chris Xu, Cornell Univ. (United States)
Ina P. Pavlova, Rice Univ. (United States)
Wendy O. Williams, Cornell Univ. (United States)
Sunish Mohanan, Cornell Univ. (United States)

© SPIE. Terms of Use
Back to Top