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Journal of Biomedical Optics • Open Access

Anisotropic light scattering of individual sickle red blood cells
Author(s): Youngchan Kim; YongKeun Park; John M. Higgins; Ramachandra Rao Dasari; Subra Suresh

Paper Abstract

We present the anisotropic light scattering of individual red blood cells (RBCs) from a patient with sickle cell disease (SCD). To measure light scattering spectra along two independent axes of elongated-shaped sickle RBCs with arbitrary orientation, we introduce the anisotropic Fourier transform light scattering (aFTLS) technique and measured both the static and dynamic anisotropic light scattering. We observed strong anisotropy in light scattering patterns of elongated-shaped sickle RBCs along its major axes using static aFTLS. Dynamic aFTLS analysis reveals the significantly altered biophysical properties in individual sickle RBCs. These results provide evidence that effective viscosity and elasticity of sickle RBCs are significantly different from those of the healthy RBCs.

Paper Details

Date Published: 5 April 2012
PDF: 4 pages
J. Biomed. Opt. 17(4) 040501 doi: 10.1117/1.JBO.17.4.040501
Published in: Journal of Biomedical Optics Volume 17, Issue 4
Show Author Affiliations
Youngchan Kim, KAIST (Korea, Republic of)
YongKeun Park, KAIST (Korea, Republic of)
John M. Higgins, Massachusetts General Hospital (United States)
Ramachandra Rao Dasari, Massachusetts Institute of Technology (United States)
Subra Suresh, Massachusetts Institute of Technology (United States)

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