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Journal of Biomedical Optics • Open Access

Influence of absorption and scattering on the quantification of fluorescence diffuse optical tomography using normalized data
Author(s): Juan Felipe Perez-Juste Abascal; Juan Aguirre; Judit Chamorro-Servent; Jorge Ripoll; Juan J. Vaquero; Manuel Desco; Martin Schweiger; Simon R. Arridge

Paper Abstract

Reconstruction algorithms for imaging fluorescence in near infrared ranges usually normalize fluorescence light with respect to excitation light. Using this approach, we investigated the influence of absorption and scattering heterogeneities on quantification accuracy when assuming a homogeneous model and explored possible reconstruction improvements by using a heterogeneous model. To do so, we created several computer-simulated phantoms: a homogeneous slab phantom (P1), slab phantoms including a region with a two- to six-fold increase in scattering (P2) and in absorption (P3), and an atlas-based mouse phantom that modeled different liver and lung scattering (P4). For P1, reconstruction with the wrong optical properties yielded quantification errors that increased almost linearly with the scattering coefficient while they were mostly negligible regarding the absorption coefficient. This observation agreed with the theoretical results. Taking the quantification of a homogeneous phantom as a reference, relative quantification errors obtained when wrongly assuming homogeneous media were in the range +41 to +94% (P2), 0.1 to −7% (P3), and −39 to +44% (P4). Using a heterogeneous model, the overall error ranged from −7 to 7%. In conclusion, this work demonstrates that assuming homogeneous media leads to noticeable quantification errors that can be improved by adopting heterogeneous models.

Paper Details

Date Published: 5 April 2012
PDF: 10 pages
J. Biomed. Opt. 17(3) 036013 doi: 10.1117/1.JBO.17.3.036013
Published in: Journal of Biomedical Optics Volume 17, Issue 3
Show Author Affiliations
Juan Felipe Perez-Juste Abascal, Univ. Carlos III de Madrid (Spain)
Juan Aguirre, Univ. Carlos III de Madrid (Spain)
Judit Chamorro-Servent, Univ. Carlos III de Madrid (Spain)
Jorge Ripoll, Univ. Carlos III de Madrid (Spain)
Juan J. Vaquero, Univ. Carlos III de Madrid (Spain)
Manuel Desco, Univ. Carlos III de Madrid (Spain)
Martin Schweiger, Univ. College London (United Kingdom)
Simon R. Arridge, Univ. College London (United Kingdom)

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