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Journal of Biomedical Optics

Autofocus evaluation for brightfield microscopy pathology
Author(s): Rafael Redondo; Gabriel Cristóbal; Gloria Bueno Garcia; Oscar Deniz; Jesus Salido; Maria del Milagro Fernandez; Juan Vidal; Juan Carlos Valdiviezo; Rodrigo Nava; Boris Escalante-Ramírez; Marcial Garcia-Rojo
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Paper Abstract

An essential and indispensable component of automated microscopy framework is the automatic focusing system, which determines the in-focus position of a given field of view by searching the maximum value of a focusing function over a range of z-axis positions. The focus function and its computation time are crucial to the accuracy and efficiency of the system. Sixteen focusing algorithms were analyzed for histological and histopathological images. In terms of accuracy, results have shown an overall high performance by most of the methods. However, we included in the evaluation study other criteria such as computational cost and focusing curve shape which are crucial for real-time applications and were used to highlight the best practices.

Paper Details

Date Published: 26 March 2012
PDF: 9 pages
J. Biomed. Opt. 17(3) 036008 doi: 10.1117/1.JBO.17.3.036008
Published in: Journal of Biomedical Optics Volume 17, Issue 3
Show Author Affiliations
Rafael Redondo, Consejo Superior de Investigaciones Científicas (Spain)
Gabriel Cristóbal, Consejo Superior de Investigaciones Científicas (Spain)
Gloria Bueno Garcia, Univ. de Castilla-La Mancha (Spain)
Oscar Deniz, Univ. de Castilla-La Mancha (Spain)
Jesus Salido, Univ. de Castilla-La Mancha (Spain)
Maria del Milagro Fernandez, Univ. de Castilla-La Mancha (Spain)
Juan Vidal, Univ. de Castilla-La Mancha (Spain)
Juan Carlos Valdiviezo, Instituto Nacional de Astrofísica, Óptica y Electrónica (Mexico)
Rodrigo Nava, Univ. Nacional Autónoma de México (Mexico)
Boris Escalante-Ramírez, Univ. Nacional Autónoma de México (Mexico)
Marcial Garcia-Rojo, Hospital General Univ. de Ciuda (Spain)

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